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1.
Arq. bras. med. vet. zootec ; 66(2): 631-634, Jan.-Apr. 2014.
Article in Portuguese | LILACS | ID: lil-709309

ABSTRACT

This study aimed to report three cases of contagious agalactia (CA) by Mycoplasma agalactiae in goat kids born with polyarthritis. The nanny goats belonging to two different herds presented clinical signs of CA during pregnancy and in parturition they were apparently healthy. The carpal articulations of the three goat kids, the tarsus articulation in one, and thigh-femoral articulation in another showed swelling, pain and impairment of the flexion-extension movements. The articular liquid was collected from two goat kids at birth and revealed a content which varied from transparent to fibrinopurulent, presenting a yellow coloring. The samples were plated on modified Hayflick. The colonies had the appearance of "fried egg" and were confirmed as being M. agalactiae by biochemical tests and 16S rRNA PCR. Blood was collected from three animals soon after birth and submitted to the indirect ELISA test for the determination of the titration of the anti- M. agalactiae antibodies. The results confirmed that the goat kids were infected during pregnancy by M. agalactiae and resulted in the birth of an offspring with clinical signs of CA being immune tolerant...


Subject(s)
Animals , Arthritis/veterinary , Infections/transmission , Mycoplasma agalactiae/isolation & purification , Ruminants , Polymerase Chain Reaction/veterinary , Maternal-Fetal Exchange/immunology
2.
Arq. bras. med. vet. zootec ; 65(5): 1394-1402, out. 2013. graf, tab
Article in English | LILACS | ID: lil-689757

ABSTRACT

This paper aims to evaluate the efficiency of three inactive vaccines against contagious agalactia prepared with samples of Mycoplasma agalactiae isolated in Brazil and different adjuvants. Vaccine 1 adsorbed with aluminum hydroxide was administered in 23 goats (Gc1) and 13 sheep (Gov1); vaccine 2 containing Montanide IMS-2215-VG was administered in 22 goats (Gc2) and 12 sheep (Gov2) and vaccine 3, containing Montanide Gel-01 was administered in 22 goats (Gc3) and 12 sheep (Gov3). All animals were negative for Ma at indirect ELISA and received two doses of 2mL each, subcutaneously, within a 21 day interval. Five animals from each species were used as control. Seventy-five days after the booster, four animals from each vaccinated group and two from the control group were challenged with 5mL of Ma culture containing 10(7)cfu/mL, orally and through immersion of the female's udder in lactation. The serological response was analyzed during vaccination days (0 and 21) and at 51, 81, 111, 141 and 171 days after vaccination. The collection and analysis of the challenged animals were conducted at the day of the challenge (D0) and 7, 14, 21, 28, 35, 42, 49 and 56 days after the challenge. The three vaccines induced the production of antibodies, having no significant statistical difference (p<0.05). Animals from groups Gc1, Gc2 and Gov2 developed higher levels of antibodies, with significant statistical difference compared to the other vaccinated group and control group (p<0.05). After the challenge, the animals from the control presented an increase in regional lymph nodes and conjunctivitis, mastitis and arthritis. In four vaccinated animals, discrete conjunctivitis and congestion of the episcleral veins was observed. It is concluded that vaccines 1 and 2 induced levels of protective antibodies in goats and sheep, sufficient for clinical protection of the animals submitted to the experimental infection, indicating its use on the prevention of contagious agalactia.


Este trabalho tem como objetivo avaliar a eficácia de três vacinas inativadas contra agalaxia contagiosa, preparadas com amostra de Mycoplasma agalactiae isolada no Brasil e diferentes adjuvantes. A vacina 1, adsorvida com hidróxido de alumínio, foi administrada em 23 caprinos (Gc1) e 13 ovinos (Gov1); a vacina 2, contendo Montanide IMS-2215-VG, foi administrada em 22 caprinos (Gc2) e 12 ovinos (Gov2); e a vacina 3, contendo Montanide Gel-01 foi administrada em 22 caprinos (Gc3) e 12 ovinos (Gov3). Todos os animais eram negativos para Ma no ELISA indireto e receberam duas doses de 2mL cada, por via subcutânea, com intervalo de 21 dias. Cinco animais de cada espécie foram utilizados como controle. Setenta e cinco dias após o reforço, quatro animais de cada grupo vacinado e dois do grupo controle foram desafiados com 5mL de cultura de Ma contendo 10(7)ufc/mL, por via oral e pela imersão dos tetos das fêmeas em lactação. A resposta sorológica foi analisada nos dias da vacinação (zero e 21) e aos 51, 81, 111, 141 e 171 dias pós-vacinação. As coletas e análises dos animais desafiados foram realizadas no dia do desafio (D0) e sete, 14, 21, 28, 35, 42, 49 e 56 dias pós-desafio. As três vacinas induziram produção de anticorpos, não havendo diferença estatisticamente significativa entre caprinos e ovinos (P>0,05). Animais dos grupos Gc1, Gc2 e Gov2 produziram níveis de anticorpos mais elevados, com diferença estatisticamente significativa em relação aos demais grupos vacinados e ao grupo controle (P<0,05). Após o desafio, os animais do grupo controle apresentaram aumento de linfonodos regionais e conjuntivite, mastite e artrite. Em quatro animais vacinados, foi observada discreta conjuntivite e congestão dos vasos episclerais. Conclui-se que as vacinas 1 e 2 induziram níveis de anticorpos protetores em caprinos e ovinos suficientes para proteção clínica dos animais submetidos à infecção experimental, podendo ser indicadas para prevenção da agalaxia contagiosa.


Subject(s)
Animals , Mycoplasma agalactiae , Mycoplasma agalactiae/chemistry , Vaccines/administration & dosage , Vaccines/analysis , Vaccines/adverse effects , Enzyme-Linked Immunosorbent Assay/adverse effects , Enzyme-Linked Immunosorbent Assay/veterinary
3.
Arq. bras. med. vet. zootec ; 60(5): 1255-1258, out. 2008. ilus, tab
Article in English | LILACS | ID: lil-500097

ABSTRACT

O objetivo deste trabalho foi verificar a ocorrência de Mycoplasma agalactiae, com o uso da reação em cadeia da polimerase (PCR), em 30 rebanhos caprinos leiteiros nas microrregiões do Cariri Ocidental e Oriental do estado da Paraíba. Das 120 amostras de leite testadas, nove (7,5 por cento) apresentaram amplificação de apenas um fragmento com 360pb. As amostras que amplificaram este fragmento estavam presentes em 6/30 (20 por cento) fazendas localizadas nos municípios de Amparo, Santo André e Gurjão.


Subject(s)
Animals , Goats , Mycoplasma Infections/epidemiology , Milk/chemistry , Mycoplasma agalactiae/isolation & purification , Polymerase Chain Reaction/methods
4.
Arq. bras. med. vet. zootec ; 59(6): 1597-1600, dez. 2007. tab
Article in Portuguese | LILACS | ID: lil-476140

ABSTRACT

This work was performed in 60 goat farms located in 15 counties of the micro regions of Western and Eastern Cariri, in Paraiba, to describe and analyze the sanitary profile of the flocks. Visits were done and the answers, obtained from questionnaires that were applied to the farmers, were used. It was observed a mortality of 20 percent in young animals in 80 percent of the farms. The presence of technical assistance in 93.3 percent in the studied farms, adoption of prophylactic calendar in 85 percent and parasitic control in 83.3 percent of the flocks were also observed


Subject(s)
Animals , Goats , Health Profile , Measures of Disease Occurrence
5.
Arq. bras. med. vet. zootec ; 52(1): 19-20, fev. 2000. tab
Article in Portuguese | LILACS | ID: lil-261100

ABSTRACT

In June of 1997 the prevalence of antibodies to bluetongue virus was between 3.94 and 4.82 per cent in 137 bovine serum samples from 12 herds in Paraíba State, Brazil. This is the first report of antibodies to bluetongue virus in Paraiba State herds


Subject(s)
Animals , Bluetongue , Cattle , Orbivirus
6.
Arq. bras. med. vet. zootec ; 51(1): 27-30, fev. 1999. ilus, tab
Article in Portuguese | LILACS | ID: lil-257137

ABSTRACT

Entre os anos de 1986 e 1996, foram estudadas lesöes cutâneas em 35 eqüinos e três muares, provenientes do município de Patos-Paraíba e regiöes circunvizinhas. As lesöes tinham localizaçäo e tamanho variados com características granulomatosas e ulceradas, com secreçäo serossangüinolenta e concreçöes amareladas de consistência firme e aspecto de coral marinho (kunkers). Para o diagnóstico definitico de pitiose utilizaram-se exames histopatológicos, cultura e identificaçäo do agente


Subject(s)
Animals , Equidae , Pityriasis/diagnosis
7.
Arq. bras. med. vet. zootec ; 50(3): 239-42, jun. 1998. tab
Article in Portuguese | LILACS | ID: lil-224102

ABSTRACT

Determinou-se a eficiência de sete vacinas comerciais contra clostridioses, que continham toxóides de Clostridium perfringens tipos C e/ou D, pelo teste de soroneutralizaçäo em camundongos a partir do "pool" de soros de coelhos imunizados. Seis vacinas produzidas no Brasil foram ineficientes em estimular níveis sorológicos de beta e épsilon antitoxinas compatíveis com os níveis de teste recomendados para controle destes produtos. Uma vacina importada apresentou títulos de anticorpos séricos superiores aos níveis mínimos exigidos de 10 e 5UI/ml para as toxinas beta e épsilon, produzidas por C. perfringens tipos C e D, respectivamente. Como controle dos testes empregou-se um toxóide bivalente padräo. Quatro dos seis toxóides de origem nacional, quando testados em caprinos, näo apresentaram níveis de antitoxinas detectáveis com os níveis de testes de L+ e L+/10 para beta e épsilon toxinas, respectivamente


Subject(s)
Animals , Clostridium perfringens/immunology , Enterotoxemia , Mice , Vaccines
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